tandem Search Results


tandem signal enchancer  (Miltenyi Biotec)
97
Miltenyi Biotec tandem signal enchancer
Tandem Signal Enchancer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tandem/Tandem+Signal+Enhancer%2C+human/bio_rxiv__64898__2026__03__21__713278-57-15-19
Average 97 stars, based on 1 article reviews
tandem signal enchancer - by Bioz Stars, 2026-11
97/100 stars
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il 12 heterodimer cdna  (Addgene inc)
93
Addgene inc il 12 heterodimer cdna
The IL-12p35 subunit is not secreted in the absence of the IL-12p40 binding partner: Development of two novel reporter systems to track the release of p35 from cells and subcellular location throughout p40-independent release. (A) Luciferase assay to detect p35NL release in the cell culture supernatant when co-expressed with p40. HEK cells were transfected with p35NL + empty vector (light red), p35NL + p40 (red), NanoLuc-only (NLO) + empty vector (light blue), or NLO + p40 (blue). Supernatants were collected 48 hours after transfection for luciferase assay (n=3). Luminescence data is represented in Raw Luminescence Units (RLUs). Functionality of p35NL as <t>an</t> <t>IL-12</t> subunit when co-expressed with p40. SMARTA transgenic T cells were activated in vitro and re-stimulated with either recombinant p40 protein, recombinant IL-12, or supernatants from HEK cells co-transfected with p35NL and p40. After 2 hours of re-stimulation, IFN-γ production by T cells was measured using intracellular cytokine staining. (B) MFI of live CD4 + CD44 + IFN-γ + SMARTA transgenic T cells populations after restimulation (n = 3). (C) Percentages of MFI of live SMARTA CD4 + CD44 + IFN-γ + transgenic T cells populations after restimulation (n = 3). (D) Representative flow plots compare the percentage of live CD4+CD44+IFNγ+ cells among groups (n = 3). Characterization of fluorescent reporter system for tracking the intracellular location of p35 through ER and Golgi compartments. (E) Murine fibroblasts (L cells) were retrovirally transduced with p35-Scarlet, Golgi-eGFP (Beta-1,4-galactosyl transferase 1), and ER-Halotag (KDEL tagged with Halotag and developed with JFX650). Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. Characterization of fluorescent reporter system for tracking the association of p35 with ER chaperone, BiP. (F) L cells were retrovirally transduced with p35-Scarlet, Golgi-eGFP, and BiP-BFP. Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. (G) Pixel intensity of each marker in p35-Scarlet/Golgi-GFP/ER-Halotag L cells along the ROI line draw in ‘MERGED (F)’. ROI line is designated by an arrow. (H) Pixel intensity of each marker in p35-Scar/Golgi-GFP/BiP-BFP L cells along the ROI line draw in ‘MERGED (G)’. ROI line is designated by an arrow. Data is presented as the mean with SEM error bars. Significant changes in luminescence in (A) were determined using an unpaired t-test. Significant changes in percentage and MFI in (B) and (C) were determined using an unpaired t-test. p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns = not significant.
Il 12 Heterodimer Cdna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tandem/Tandem+p40p35+(Plasmid+%23108665)/bio_rxiv__64898__2026__01__28__702376-272-16-19
Average 93 stars, based on 1 article reviews
il 12 heterodimer cdna - by Bioz Stars, 2026-11
93/100 stars
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cellchecktm canine str profiling test  (IDEXX)
97
IDEXX cellchecktm canine str profiling test
The IL-12p35 subunit is not secreted in the absence of the IL-12p40 binding partner: Development of two novel reporter systems to track the release of p35 from cells and subcellular location throughout p40-independent release. (A) Luciferase assay to detect p35NL release in the cell culture supernatant when co-expressed with p40. HEK cells were transfected with p35NL + empty vector (light red), p35NL + p40 (red), NanoLuc-only (NLO) + empty vector (light blue), or NLO + p40 (blue). Supernatants were collected 48 hours after transfection for luciferase assay (n=3). Luminescence data is represented in Raw Luminescence Units (RLUs). Functionality of p35NL as <t>an</t> <t>IL-12</t> subunit when co-expressed with p40. SMARTA transgenic T cells were activated in vitro and re-stimulated with either recombinant p40 protein, recombinant IL-12, or supernatants from HEK cells co-transfected with p35NL and p40. After 2 hours of re-stimulation, IFN-γ production by T cells was measured using intracellular cytokine staining. (B) MFI of live CD4 + CD44 + IFN-γ + SMARTA transgenic T cells populations after restimulation (n = 3). (C) Percentages of MFI of live SMARTA CD4 + CD44 + IFN-γ + transgenic T cells populations after restimulation (n = 3). (D) Representative flow plots compare the percentage of live CD4+CD44+IFNγ+ cells among groups (n = 3). Characterization of fluorescent reporter system for tracking the intracellular location of p35 through ER and Golgi compartments. (E) Murine fibroblasts (L cells) were retrovirally transduced with p35-Scarlet, Golgi-eGFP (Beta-1,4-galactosyl transferase 1), and ER-Halotag (KDEL tagged with Halotag and developed with JFX650). Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. Characterization of fluorescent reporter system for tracking the association of p35 with ER chaperone, BiP. (F) L cells were retrovirally transduced with p35-Scarlet, Golgi-eGFP, and BiP-BFP. Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. (G) Pixel intensity of each marker in p35-Scarlet/Golgi-GFP/ER-Halotag L cells along the ROI line draw in ‘MERGED (F)’. ROI line is designated by an arrow. (H) Pixel intensity of each marker in p35-Scar/Golgi-GFP/BiP-BFP L cells along the ROI line draw in ‘MERGED (G)’. ROI line is designated by an arrow. Data is presented as the mean with SEM error bars. Significant changes in luminescence in (A) were determined using an unpaired t-test. Significant changes in percentage and MFI in (B) and (C) were determined using an unpaired t-test. p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns = not significant.
Cellchecktm Canine Str Profiling Test, supplied by IDEXX, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tandem/CellCheck/10__1186_slash_s44356___025___00049___w-123-12-17
Average 97 stars, based on 1 article reviews
cellchecktm canine str profiling test - by Bioz Stars, 2026-11
97/100 stars
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acylcarnitines tandemmass spectrometry kit  (Revvity)
91
Revvity acylcarnitines tandemmass spectrometry kit
The IL-12p35 subunit is not secreted in the absence of the IL-12p40 binding partner: Development of two novel reporter systems to track the release of p35 from cells and subcellular location throughout p40-independent release. (A) Luciferase assay to detect p35NL release in the cell culture supernatant when co-expressed with p40. HEK cells were transfected with p35NL + empty vector (light red), p35NL + p40 (red), NanoLuc-only (NLO) + empty vector (light blue), or NLO + p40 (blue). Supernatants were collected 48 hours after transfection for luciferase assay (n=3). Luminescence data is represented in Raw Luminescence Units (RLUs). Functionality of p35NL as <t>an</t> <t>IL-12</t> subunit when co-expressed with p40. SMARTA transgenic T cells were activated in vitro and re-stimulated with either recombinant p40 protein, recombinant IL-12, or supernatants from HEK cells co-transfected with p35NL and p40. After 2 hours of re-stimulation, IFN-γ production by T cells was measured using intracellular cytokine staining. (B) MFI of live CD4 + CD44 + IFN-γ + SMARTA transgenic T cells populations after restimulation (n = 3). (C) Percentages of MFI of live SMARTA CD4 + CD44 + IFN-γ + transgenic T cells populations after restimulation (n = 3). (D) Representative flow plots compare the percentage of live CD4+CD44+IFNγ+ cells among groups (n = 3). Characterization of fluorescent reporter system for tracking the intracellular location of p35 through ER and Golgi compartments. (E) Murine fibroblasts (L cells) were retrovirally transduced with p35-Scarlet, Golgi-eGFP (Beta-1,4-galactosyl transferase 1), and ER-Halotag (KDEL tagged with Halotag and developed with JFX650). Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. Characterization of fluorescent reporter system for tracking the association of p35 with ER chaperone, BiP. (F) L cells were retrovirally transduced with p35-Scarlet, Golgi-eGFP, and BiP-BFP. Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. (G) Pixel intensity of each marker in p35-Scarlet/Golgi-GFP/ER-Halotag L cells along the ROI line draw in ‘MERGED (F)’. ROI line is designated by an arrow. (H) Pixel intensity of each marker in p35-Scar/Golgi-GFP/BiP-BFP L cells along the ROI line draw in ‘MERGED (G)’. ROI line is designated by an arrow. Data is presented as the mean with SEM error bars. Significant changes in luminescence in (A) were determined using an unpaired t-test. Significant changes in percentage and MFI in (B) and (C) were determined using an unpaired t-test. p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns = not significant.
Acylcarnitines Tandemmass Spectrometry Kit, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tandem/NeoGram+AAAC+Tandem+Mass+Spectrometry+kit/pmc09865855-298-15-19
Average 91 stars, based on 1 article reviews
acylcarnitines tandemmass spectrometry kit - by Bioz Stars, 2026-11
91/100 stars
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ha p62 addgene  (Addgene inc)
91
Addgene inc ha p62 addgene
The IL-12p35 subunit is not secreted in the absence of the IL-12p40 binding partner: Development of two novel reporter systems to track the release of p35 from cells and subcellular location throughout p40-independent release. (A) Luciferase assay to detect p35NL release in the cell culture supernatant when co-expressed with p40. HEK cells were transfected with p35NL + empty vector (light red), p35NL + p40 (red), NanoLuc-only (NLO) + empty vector (light blue), or NLO + p40 (blue). Supernatants were collected 48 hours after transfection for luciferase assay (n=3). Luminescence data is represented in Raw Luminescence Units (RLUs). Functionality of p35NL as <t>an</t> <t>IL-12</t> subunit when co-expressed with p40. SMARTA transgenic T cells were activated in vitro and re-stimulated with either recombinant p40 protein, recombinant IL-12, or supernatants from HEK cells co-transfected with p35NL and p40. After 2 hours of re-stimulation, IFN-γ production by T cells was measured using intracellular cytokine staining. (B) MFI of live CD4 + CD44 + IFN-γ + SMARTA transgenic T cells populations after restimulation (n = 3). (C) Percentages of MFI of live SMARTA CD4 + CD44 + IFN-γ + transgenic T cells populations after restimulation (n = 3). (D) Representative flow plots compare the percentage of live CD4+CD44+IFNγ+ cells among groups (n = 3). Characterization of fluorescent reporter system for tracking the intracellular location of p35 through ER and Golgi compartments. (E) Murine fibroblasts (L cells) were retrovirally transduced with p35-Scarlet, Golgi-eGFP (Beta-1,4-galactosyl transferase 1), and ER-Halotag (KDEL tagged with Halotag and developed with JFX650). Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. Characterization of fluorescent reporter system for tracking the association of p35 with ER chaperone, BiP. (F) L cells were retrovirally transduced with p35-Scarlet, Golgi-eGFP, and BiP-BFP. Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. (G) Pixel intensity of each marker in p35-Scarlet/Golgi-GFP/ER-Halotag L cells along the ROI line draw in ‘MERGED (F)’. ROI line is designated by an arrow. (H) Pixel intensity of each marker in p35-Scar/Golgi-GFP/BiP-BFP L cells along the ROI line draw in ‘MERGED (G)’. ROI line is designated by an arrow. Data is presented as the mean with SEM error bars. Significant changes in luminescence in (A) were determined using an unpaired t-test. Significant changes in percentage and MFI in (B) and (C) were determined using an unpaired t-test. p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns = not significant.
Ha P62 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tandem/pDEST-CMV-N-Tandem-mCherry-EGFP+(Plasmid+%23123216)/pm37595580-709-114-115
Average 91 stars, based on 1 article reviews
ha p62 addgene - by Bioz Stars, 2026-11
91/100 stars
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da transporter blockers  (MedChemExpress)
94
MedChemExpress da transporter blockers
The IL-12p35 subunit is not secreted in the absence of the IL-12p40 binding partner: Development of two novel reporter systems to track the release of p35 from cells and subcellular location throughout p40-independent release. (A) Luciferase assay to detect p35NL release in the cell culture supernatant when co-expressed with p40. HEK cells were transfected with p35NL + empty vector (light red), p35NL + p40 (red), NanoLuc-only (NLO) + empty vector (light blue), or NLO + p40 (blue). Supernatants were collected 48 hours after transfection for luciferase assay (n=3). Luminescence data is represented in Raw Luminescence Units (RLUs). Functionality of p35NL as <t>an</t> <t>IL-12</t> subunit when co-expressed with p40. SMARTA transgenic T cells were activated in vitro and re-stimulated with either recombinant p40 protein, recombinant IL-12, or supernatants from HEK cells co-transfected with p35NL and p40. After 2 hours of re-stimulation, IFN-γ production by T cells was measured using intracellular cytokine staining. (B) MFI of live CD4 + CD44 + IFN-γ + SMARTA transgenic T cells populations after restimulation (n = 3). (C) Percentages of MFI of live SMARTA CD4 + CD44 + IFN-γ + transgenic T cells populations after restimulation (n = 3). (D) Representative flow plots compare the percentage of live CD4+CD44+IFNγ+ cells among groups (n = 3). Characterization of fluorescent reporter system for tracking the intracellular location of p35 through ER and Golgi compartments. (E) Murine fibroblasts (L cells) were retrovirally transduced with p35-Scarlet, Golgi-eGFP (Beta-1,4-galactosyl transferase 1), and ER-Halotag (KDEL tagged with Halotag and developed with JFX650). Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. Characterization of fluorescent reporter system for tracking the association of p35 with ER chaperone, BiP. (F) L cells were retrovirally transduced with p35-Scarlet, Golgi-eGFP, and BiP-BFP. Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. (G) Pixel intensity of each marker in p35-Scarlet/Golgi-GFP/ER-Halotag L cells along the ROI line draw in ‘MERGED (F)’. ROI line is designated by an arrow. (H) Pixel intensity of each marker in p35-Scar/Golgi-GFP/BiP-BFP L cells along the ROI line draw in ‘MERGED (G)’. ROI line is designated by an arrow. Data is presented as the mean with SEM error bars. Significant changes in luminescence in (A) were determined using an unpaired t-test. Significant changes in percentage and MFI in (B) and (C) were determined using an unpaired t-test. p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns = not significant.
Da Transporter Blockers, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tandem/Dopamine+Transporter+Antibody/pmc06141656-67-2-12
Average 94 stars, based on 1 article reviews
da transporter blockers - by Bioz Stars, 2026-11
94/100 stars
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maldi-tandem ms  (Micromass UK Limited)
90
Micromass UK Limited maldi-tandem ms
The IL-12p35 subunit is not secreted in the absence of the IL-12p40 binding partner: Development of two novel reporter systems to track the release of p35 from cells and subcellular location throughout p40-independent release. (A) Luciferase assay to detect p35NL release in the cell culture supernatant when co-expressed with p40. HEK cells were transfected with p35NL + empty vector (light red), p35NL + p40 (red), NanoLuc-only (NLO) + empty vector (light blue), or NLO + p40 (blue). Supernatants were collected 48 hours after transfection for luciferase assay (n=3). Luminescence data is represented in Raw Luminescence Units (RLUs). Functionality of p35NL as <t>an</t> <t>IL-12</t> subunit when co-expressed with p40. SMARTA transgenic T cells were activated in vitro and re-stimulated with either recombinant p40 protein, recombinant IL-12, or supernatants from HEK cells co-transfected with p35NL and p40. After 2 hours of re-stimulation, IFN-γ production by T cells was measured using intracellular cytokine staining. (B) MFI of live CD4 + CD44 + IFN-γ + SMARTA transgenic T cells populations after restimulation (n = 3). (C) Percentages of MFI of live SMARTA CD4 + CD44 + IFN-γ + transgenic T cells populations after restimulation (n = 3). (D) Representative flow plots compare the percentage of live CD4+CD44+IFNγ+ cells among groups (n = 3). Characterization of fluorescent reporter system for tracking the intracellular location of p35 through ER and Golgi compartments. (E) Murine fibroblasts (L cells) were retrovirally transduced with p35-Scarlet, Golgi-eGFP (Beta-1,4-galactosyl transferase 1), and ER-Halotag (KDEL tagged with Halotag and developed with JFX650). Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. Characterization of fluorescent reporter system for tracking the association of p35 with ER chaperone, BiP. (F) L cells were retrovirally transduced with p35-Scarlet, Golgi-eGFP, and BiP-BFP. Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. (G) Pixel intensity of each marker in p35-Scarlet/Golgi-GFP/ER-Halotag L cells along the ROI line draw in ‘MERGED (F)’. ROI line is designated by an arrow. (H) Pixel intensity of each marker in p35-Scar/Golgi-GFP/BiP-BFP L cells along the ROI line draw in ‘MERGED (G)’. ROI line is designated by an arrow. Data is presented as the mean with SEM error bars. Significant changes in luminescence in (A) were determined using an unpaired t-test. Significant changes in percentage and MFI in (B) and (C) were determined using an unpaired t-test. p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns = not significant.
Maldi Tandem Ms, supplied by Micromass UK Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tandem/maldi+tandem+ms/10__1042_slash_bj20051316-103-11-21
Average 90 stars, based on 1 article reviews
maldi-tandem ms - by Bioz Stars, 2026-11
90/100 stars
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short tandem repeats-pcr analysis  (Microsynth ag)
90
Microsynth ag short tandem repeats-pcr analysis
The IL-12p35 subunit is not secreted in the absence of the IL-12p40 binding partner: Development of two novel reporter systems to track the release of p35 from cells and subcellular location throughout p40-independent release. (A) Luciferase assay to detect p35NL release in the cell culture supernatant when co-expressed with p40. HEK cells were transfected with p35NL + empty vector (light red), p35NL + p40 (red), NanoLuc-only (NLO) + empty vector (light blue), or NLO + p40 (blue). Supernatants were collected 48 hours after transfection for luciferase assay (n=3). Luminescence data is represented in Raw Luminescence Units (RLUs). Functionality of p35NL as <t>an</t> <t>IL-12</t> subunit when co-expressed with p40. SMARTA transgenic T cells were activated in vitro and re-stimulated with either recombinant p40 protein, recombinant IL-12, or supernatants from HEK cells co-transfected with p35NL and p40. After 2 hours of re-stimulation, IFN-γ production by T cells was measured using intracellular cytokine staining. (B) MFI of live CD4 + CD44 + IFN-γ + SMARTA transgenic T cells populations after restimulation (n = 3). (C) Percentages of MFI of live SMARTA CD4 + CD44 + IFN-γ + transgenic T cells populations after restimulation (n = 3). (D) Representative flow plots compare the percentage of live CD4+CD44+IFNγ+ cells among groups (n = 3). Characterization of fluorescent reporter system for tracking the intracellular location of p35 through ER and Golgi compartments. (E) Murine fibroblasts (L cells) were retrovirally transduced with p35-Scarlet, Golgi-eGFP (Beta-1,4-galactosyl transferase 1), and ER-Halotag (KDEL tagged with Halotag and developed with JFX650). Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. Characterization of fluorescent reporter system for tracking the association of p35 with ER chaperone, BiP. (F) L cells were retrovirally transduced with p35-Scarlet, Golgi-eGFP, and BiP-BFP. Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. (G) Pixel intensity of each marker in p35-Scarlet/Golgi-GFP/ER-Halotag L cells along the ROI line draw in ‘MERGED (F)’. ROI line is designated by an arrow. (H) Pixel intensity of each marker in p35-Scar/Golgi-GFP/BiP-BFP L cells along the ROI line draw in ‘MERGED (G)’. ROI line is designated by an arrow. Data is presented as the mean with SEM error bars. Significant changes in luminescence in (A) were determined using an unpaired t-test. Significant changes in percentage and MFI in (B) and (C) were determined using an unpaired t-test. p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns = not significant.
Short Tandem Repeats Pcr Analysis, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tandem/short+tandem+repeats+pcr+analysis/10__1158_slash_1078___0432__ccr___21___2761-33-20-25
Average 90 stars, based on 1 article reviews
short tandem repeats-pcr analysis - by Bioz Stars, 2026-11
90/100 stars
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tandem-seat microlight aircraft rans s-7 courier  (Microlight Corporation)
90
Microlight Corporation tandem-seat microlight aircraft rans s-7 courier
The IL-12p35 subunit is not secreted in the absence of the IL-12p40 binding partner: Development of two novel reporter systems to track the release of p35 from cells and subcellular location throughout p40-independent release. (A) Luciferase assay to detect p35NL release in the cell culture supernatant when co-expressed with p40. HEK cells were transfected with p35NL + empty vector (light red), p35NL + p40 (red), NanoLuc-only (NLO) + empty vector (light blue), or NLO + p40 (blue). Supernatants were collected 48 hours after transfection for luciferase assay (n=3). Luminescence data is represented in Raw Luminescence Units (RLUs). Functionality of p35NL as <t>an</t> <t>IL-12</t> subunit when co-expressed with p40. SMARTA transgenic T cells were activated in vitro and re-stimulated with either recombinant p40 protein, recombinant IL-12, or supernatants from HEK cells co-transfected with p35NL and p40. After 2 hours of re-stimulation, IFN-γ production by T cells was measured using intracellular cytokine staining. (B) MFI of live CD4 + CD44 + IFN-γ + SMARTA transgenic T cells populations after restimulation (n = 3). (C) Percentages of MFI of live SMARTA CD4 + CD44 + IFN-γ + transgenic T cells populations after restimulation (n = 3). (D) Representative flow plots compare the percentage of live CD4+CD44+IFNγ+ cells among groups (n = 3). Characterization of fluorescent reporter system for tracking the intracellular location of p35 through ER and Golgi compartments. (E) Murine fibroblasts (L cells) were retrovirally transduced with p35-Scarlet, Golgi-eGFP (Beta-1,4-galactosyl transferase 1), and ER-Halotag (KDEL tagged with Halotag and developed with JFX650). Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. Characterization of fluorescent reporter system for tracking the association of p35 with ER chaperone, BiP. (F) L cells were retrovirally transduced with p35-Scarlet, Golgi-eGFP, and BiP-BFP. Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. (G) Pixel intensity of each marker in p35-Scarlet/Golgi-GFP/ER-Halotag L cells along the ROI line draw in ‘MERGED (F)’. ROI line is designated by an arrow. (H) Pixel intensity of each marker in p35-Scar/Golgi-GFP/BiP-BFP L cells along the ROI line draw in ‘MERGED (G)’. ROI line is designated by an arrow. Data is presented as the mean with SEM error bars. Significant changes in luminescence in (A) were determined using an unpaired t-test. Significant changes in percentage and MFI in (B) and (C) were determined using an unpaired t-test. p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns = not significant.
Tandem Seat Microlight Aircraft Rans S 7 Courier, supplied by Microlight Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tandem/tandem+seat+microlight+aircraft+rans+s+7+courier/10__3354_slash_esr01397-48-8-9
Average 90 stars, based on 1 article reviews
tandem-seat microlight aircraft rans s-7 courier - by Bioz Stars, 2026-11
90/100 stars
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x!tandem  (Genentech inc)
90
Genentech inc x!tandem
The IL-12p35 subunit is not secreted in the absence of the IL-12p40 binding partner: Development of two novel reporter systems to track the release of p35 from cells and subcellular location throughout p40-independent release. (A) Luciferase assay to detect p35NL release in the cell culture supernatant when co-expressed with p40. HEK cells were transfected with p35NL + empty vector (light red), p35NL + p40 (red), NanoLuc-only (NLO) + empty vector (light blue), or NLO + p40 (blue). Supernatants were collected 48 hours after transfection for luciferase assay (n=3). Luminescence data is represented in Raw Luminescence Units (RLUs). Functionality of p35NL as <t>an</t> <t>IL-12</t> subunit when co-expressed with p40. SMARTA transgenic T cells were activated in vitro and re-stimulated with either recombinant p40 protein, recombinant IL-12, or supernatants from HEK cells co-transfected with p35NL and p40. After 2 hours of re-stimulation, IFN-γ production by T cells was measured using intracellular cytokine staining. (B) MFI of live CD4 + CD44 + IFN-γ + SMARTA transgenic T cells populations after restimulation (n = 3). (C) Percentages of MFI of live SMARTA CD4 + CD44 + IFN-γ + transgenic T cells populations after restimulation (n = 3). (D) Representative flow plots compare the percentage of live CD4+CD44+IFNγ+ cells among groups (n = 3). Characterization of fluorescent reporter system for tracking the intracellular location of p35 through ER and Golgi compartments. (E) Murine fibroblasts (L cells) were retrovirally transduced with p35-Scarlet, Golgi-eGFP (Beta-1,4-galactosyl transferase 1), and ER-Halotag (KDEL tagged with Halotag and developed with JFX650). Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. Characterization of fluorescent reporter system for tracking the association of p35 with ER chaperone, BiP. (F) L cells were retrovirally transduced with p35-Scarlet, Golgi-eGFP, and BiP-BFP. Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. (G) Pixel intensity of each marker in p35-Scarlet/Golgi-GFP/ER-Halotag L cells along the ROI line draw in ‘MERGED (F)’. ROI line is designated by an arrow. (H) Pixel intensity of each marker in p35-Scar/Golgi-GFP/BiP-BFP L cells along the ROI line draw in ‘MERGED (G)’. ROI line is designated by an arrow. Data is presented as the mean with SEM error bars. Significant changes in luminescence in (A) were determined using an unpaired t-test. Significant changes in percentage and MFI in (B) and (C) were determined using an unpaired t-test. p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns = not significant.
X!Tandem, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tandem/x+tandem/pmc03134881-393-12-5
Average 90 stars, based on 1 article reviews
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tandem repeat finder program  (Biomath GmbH)
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The IL-12p35 subunit is not secreted in the absence of the IL-12p40 binding partner: Development of two novel reporter systems to track the release of p35 from cells and subcellular location throughout p40-independent release. (A) Luciferase assay to detect p35NL release in the cell culture supernatant when co-expressed with p40. HEK cells were transfected with p35NL + empty vector (light red), p35NL + p40 (red), NanoLuc-only (NLO) + empty vector (light blue), or NLO + p40 (blue). Supernatants were collected 48 hours after transfection for luciferase assay (n=3). Luminescence data is represented in Raw Luminescence Units (RLUs). Functionality of p35NL as <t>an</t> <t>IL-12</t> subunit when co-expressed with p40. SMARTA transgenic T cells were activated in vitro and re-stimulated with either recombinant p40 protein, recombinant IL-12, or supernatants from HEK cells co-transfected with p35NL and p40. After 2 hours of re-stimulation, IFN-γ production by T cells was measured using intracellular cytokine staining. (B) MFI of live CD4 + CD44 + IFN-γ + SMARTA transgenic T cells populations after restimulation (n = 3). (C) Percentages of MFI of live SMARTA CD4 + CD44 + IFN-γ + transgenic T cells populations after restimulation (n = 3). (D) Representative flow plots compare the percentage of live CD4+CD44+IFNγ+ cells among groups (n = 3). Characterization of fluorescent reporter system for tracking the intracellular location of p35 through ER and Golgi compartments. (E) Murine fibroblasts (L cells) were retrovirally transduced with p35-Scarlet, Golgi-eGFP (Beta-1,4-galactosyl transferase 1), and ER-Halotag (KDEL tagged with Halotag and developed with JFX650). Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. Characterization of fluorescent reporter system for tracking the association of p35 with ER chaperone, BiP. (F) L cells were retrovirally transduced with p35-Scarlet, Golgi-eGFP, and BiP-BFP. Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. (G) Pixel intensity of each marker in p35-Scarlet/Golgi-GFP/ER-Halotag L cells along the ROI line draw in ‘MERGED (F)’. ROI line is designated by an arrow. (H) Pixel intensity of each marker in p35-Scar/Golgi-GFP/BiP-BFP L cells along the ROI line draw in ‘MERGED (G)’. ROI line is designated by an arrow. Data is presented as the mean with SEM error bars. Significant changes in luminescence in (A) were determined using an unpaired t-test. Significant changes in percentage and MFI in (B) and (C) were determined using an unpaired t-test. p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns = not significant.
Tandem Repeat Finder Program, supplied by Biomath GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The IL-12p35 subunit is not secreted in the absence of the IL-12p40 binding partner: Development of two novel reporter systems to track the release of p35 from cells and subcellular location throughout p40-independent release. (A) Luciferase assay to detect p35NL release in the cell culture supernatant when co-expressed with p40. HEK cells were transfected with p35NL + empty vector (light red), p35NL + p40 (red), NanoLuc-only (NLO) + empty vector (light blue), or NLO + p40 (blue). Supernatants were collected 48 hours after transfection for luciferase assay (n=3). Luminescence data is represented in Raw Luminescence Units (RLUs). Functionality of p35NL as <t>an</t> <t>IL-12</t> subunit when co-expressed with p40. SMARTA transgenic T cells were activated in vitro and re-stimulated with either recombinant p40 protein, recombinant IL-12, or supernatants from HEK cells co-transfected with p35NL and p40. After 2 hours of re-stimulation, IFN-γ production by T cells was measured using intracellular cytokine staining. (B) MFI of live CD4 + CD44 + IFN-γ + SMARTA transgenic T cells populations after restimulation (n = 3). (C) Percentages of MFI of live SMARTA CD4 + CD44 + IFN-γ + transgenic T cells populations after restimulation (n = 3). (D) Representative flow plots compare the percentage of live CD4+CD44+IFNγ+ cells among groups (n = 3). Characterization of fluorescent reporter system for tracking the intracellular location of p35 through ER and Golgi compartments. (E) Murine fibroblasts (L cells) were retrovirally transduced with p35-Scarlet, Golgi-eGFP (Beta-1,4-galactosyl transferase 1), and ER-Halotag (KDEL tagged with Halotag and developed with JFX650). Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. Characterization of fluorescent reporter system for tracking the association of p35 with ER chaperone, BiP. (F) L cells were retrovirally transduced with p35-Scarlet, Golgi-eGFP, and BiP-BFP. Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. (G) Pixel intensity of each marker in p35-Scarlet/Golgi-GFP/ER-Halotag L cells along the ROI line draw in ‘MERGED (F)’. ROI line is designated by an arrow. (H) Pixel intensity of each marker in p35-Scar/Golgi-GFP/BiP-BFP L cells along the ROI line draw in ‘MERGED (G)’. ROI line is designated by an arrow. Data is presented as the mean with SEM error bars. Significant changes in luminescence in (A) were determined using an unpaired t-test. Significant changes in percentage and MFI in (B) and (C) were determined using an unpaired t-test. p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns = not significant.
Mascot Tandem Mass Spectrometry Search Engine, supplied by BioWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The IL-12p35 subunit is not secreted in the absence of the IL-12p40 binding partner: Development of two novel reporter systems to track the release of p35 from cells and subcellular location throughout p40-independent release. (A) Luciferase assay to detect p35NL release in the cell culture supernatant when co-expressed with p40. HEK cells were transfected with p35NL + empty vector (light red), p35NL + p40 (red), NanoLuc-only (NLO) + empty vector (light blue), or NLO + p40 (blue). Supernatants were collected 48 hours after transfection for luciferase assay (n=3). Luminescence data is represented in Raw Luminescence Units (RLUs). Functionality of p35NL as an IL-12 subunit when co-expressed with p40. SMARTA transgenic T cells were activated in vitro and re-stimulated with either recombinant p40 protein, recombinant IL-12, or supernatants from HEK cells co-transfected with p35NL and p40. After 2 hours of re-stimulation, IFN-γ production by T cells was measured using intracellular cytokine staining. (B) MFI of live CD4 + CD44 + IFN-γ + SMARTA transgenic T cells populations after restimulation (n = 3). (C) Percentages of MFI of live SMARTA CD4 + CD44 + IFN-γ + transgenic T cells populations after restimulation (n = 3). (D) Representative flow plots compare the percentage of live CD4+CD44+IFNγ+ cells among groups (n = 3). Characterization of fluorescent reporter system for tracking the intracellular location of p35 through ER and Golgi compartments. (E) Murine fibroblasts (L cells) were retrovirally transduced with p35-Scarlet, Golgi-eGFP (Beta-1,4-galactosyl transferase 1), and ER-Halotag (KDEL tagged with Halotag and developed with JFX650). Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. Characterization of fluorescent reporter system for tracking the association of p35 with ER chaperone, BiP. (F) L cells were retrovirally transduced with p35-Scarlet, Golgi-eGFP, and BiP-BFP. Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. (G) Pixel intensity of each marker in p35-Scarlet/Golgi-GFP/ER-Halotag L cells along the ROI line draw in ‘MERGED (F)’. ROI line is designated by an arrow. (H) Pixel intensity of each marker in p35-Scar/Golgi-GFP/BiP-BFP L cells along the ROI line draw in ‘MERGED (G)’. ROI line is designated by an arrow. Data is presented as the mean with SEM error bars. Significant changes in luminescence in (A) were determined using an unpaired t-test. Significant changes in percentage and MFI in (B) and (C) were determined using an unpaired t-test. p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns = not significant.

Journal: bioRxiv

Article Title: The active secretion of a subunit of IL-12 by tissue cells is regulated by Valosin-Containing Protein and intracellular calcium redistribution

doi: 10.64898/2026.01.28.702376

Figure Lengend Snippet: The IL-12p35 subunit is not secreted in the absence of the IL-12p40 binding partner: Development of two novel reporter systems to track the release of p35 from cells and subcellular location throughout p40-independent release. (A) Luciferase assay to detect p35NL release in the cell culture supernatant when co-expressed with p40. HEK cells were transfected with p35NL + empty vector (light red), p35NL + p40 (red), NanoLuc-only (NLO) + empty vector (light blue), or NLO + p40 (blue). Supernatants were collected 48 hours after transfection for luciferase assay (n=3). Luminescence data is represented in Raw Luminescence Units (RLUs). Functionality of p35NL as an IL-12 subunit when co-expressed with p40. SMARTA transgenic T cells were activated in vitro and re-stimulated with either recombinant p40 protein, recombinant IL-12, or supernatants from HEK cells co-transfected with p35NL and p40. After 2 hours of re-stimulation, IFN-γ production by T cells was measured using intracellular cytokine staining. (B) MFI of live CD4 + CD44 + IFN-γ + SMARTA transgenic T cells populations after restimulation (n = 3). (C) Percentages of MFI of live SMARTA CD4 + CD44 + IFN-γ + transgenic T cells populations after restimulation (n = 3). (D) Representative flow plots compare the percentage of live CD4+CD44+IFNγ+ cells among groups (n = 3). Characterization of fluorescent reporter system for tracking the intracellular location of p35 through ER and Golgi compartments. (E) Murine fibroblasts (L cells) were retrovirally transduced with p35-Scarlet, Golgi-eGFP (Beta-1,4-galactosyl transferase 1), and ER-Halotag (KDEL tagged with Halotag and developed with JFX650). Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. Characterization of fluorescent reporter system for tracking the association of p35 with ER chaperone, BiP. (F) L cells were retrovirally transduced with p35-Scarlet, Golgi-eGFP, and BiP-BFP. Images are representative of steady-state cells during live cell fluorescent imaging at 100X. White scale bars correspond to 10µm. (G) Pixel intensity of each marker in p35-Scarlet/Golgi-GFP/ER-Halotag L cells along the ROI line draw in ‘MERGED (F)’. ROI line is designated by an arrow. (H) Pixel intensity of each marker in p35-Scar/Golgi-GFP/BiP-BFP L cells along the ROI line draw in ‘MERGED (G)’. ROI line is designated by an arrow. Data is presented as the mean with SEM error bars. Significant changes in luminescence in (A) were determined using an unpaired t-test. Significant changes in percentage and MFI in (B) and (C) were determined using an unpaired t-test. p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns = not significant.

Article Snippet: The p35-Scarlet (p35-Scar) construct was generated by amplifying murine p35 cDNA from a plasmid containing the IL-12 heterodimer cDNA (Addgene #108665) and cloning into MIGR1-GFP with a Myc-mScarlet cDNA fragment amplified from a previous plasmid using InFusion cloning.

Techniques: Binding Assay, Luciferase, Cell Culture, Transfection, Plasmid Preparation, Transgenic Assay, In Vitro, Recombinant, Staining, Transduction, Imaging, Marker